Contact an Account ManagerSimultaneous determination of nine intense sweeteners in foodstuffs by high performance liquid chromatography and evaporative light scattering detection - Development and single-laboratory validation
Summary
Type: HPLC
Application #: 122120
Detection:
Conditions: Eluent A: methanol – buffer solution* – acetone (69:24:7, v/v/v)
Eluent B: methanol – buffer solution* – acetone (11:82:7, v/v/v)
* 4 mL formic acid in 5 L water adjusted to pH 4.5 with ca. 12.5 mL triethylamine
Gradient: 0 % A for 4 min, 0–53 % A in 7 min, 53–100 % in 12 min, 100 % A for 1 min, 100-0 % A in 2 min, 0 % A for 10 min
Flow rate: 0.5 mL/min
Temperature: ambient
Injection volume: 8 µL
Authors: Wasik, A. et al.
Source: J. Chromatogr. A 1157 (2007), 187–196
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